Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts

  • Juergen LiebermannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)


Cryopreservation is one of the keystones in clinical infertility treatment. Especially vitrification has become a well-established and widely used routine procedure that allows important expansion of therapeutic strategies when in vitro fertilization (IVF) is used to treat infertility. Vitrification of human blastocysts allows us to maximize the potential for conception from any one in vitro fertilization cycle and prevents wastage of embryos. This goes even further toward to best utilize a patient’s supernumerary oocytes after retrieval, maximizing the use of embryos from a single stimulation cycle. The technology may even be used to eliminate fresh embryo transfers for reasons of convenience, uterine receptivity, fertility preservation, preimplantation genetic diagnosis, or emergency management. In this chapter, the application of vitrification technology for cryopreserving human blastocyst will be revealed through step-by-step protocols. The results that are presented using the described protocols underscore the robustness of the vitrification technology for embryo cryopreservation.

Key words

Human blastocyst Cryopreservation Embryo transfer Cryostorage Vitrification 



The author wants to thank the Fertility Centers of Illinois (FCI) and the embryologists at the FCI IVF Laboratory River North (Elissa Pelts, BS; Jill Matthews, BS; Sara Sanchez, BS; Rebecca Brohammer, BS; Yuri Wagner, BS; and Ewelina Pawlowska, MS) for their invaluable contributions and support in pushing vitrification to become our standard protocol for cryopreservation of human oocytes and blastocysts within our program since 2004.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Fertility Centers of IllinoisChicagoUSA

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