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A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries

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Polyadenylation in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1255))

Abstract

High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.

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Acknowledgements

This work was supported by the National Science Foundation (awards IOS-0817818 and MCB-1243849). The author thanks the staff of AGTC for many helpful discussions and for much patience, and Carol Von Lanken for excellent technical and administrative assistance.

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Authors and Affiliations

  1. Department of Plant and Soil Sciences, University of Kentucky, 301A Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546-0312, USA

    Arthur G. Hunt

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  1. Arthur G. Hunt
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Correspondence to Arthur G. Hunt .

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Editors and Affiliations

  1. Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, USA

    Arthur G. Hunt

  2. Department of Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Miami University, Oxford, OH, USA and Xiamen University, Xiamen, Fujian, Fujian, China

    Qingshun Quinn Li

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Hunt, A.G. (2015). A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries. In: Hunt, A., Li, Q. (eds) Polyadenylation in Plants. Methods in Molecular Biology, vol 1255. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2175-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-2175-1_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2174-4

  • Online ISBN: 978-1-4939-2175-1

  • eBook Packages: Springer Protocols

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