Abstract
Nuclear architecture is highly concerted including the organization of chromosome territories and distinct nuclear bodies , such as nucleoli , Cajal bodies , nuclear speckles of splicing factors, and promyelocytic leukemia nuclear bodies , among others. The organization of such nuclear compartments is very dynamic and may represent a sensitive indicator of the functional status of the cell . Here, we describe methodologies that allow isolating discrete cell populations from the brain and the fine observation of nuclear signs that could be insightful predictors of an early neuronal injury in a wide range of neurodegenerative disorders . The tools here described may be of use for the early detection of pre-degenerative processes in neurodegenerative diseases and for validating novel rescue strategies.
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Baltanás, F.C., Valero, J., Alonso, J.R., Berciano, M.T., Lafarga, M. (2015). Nuclear Signs of Pre-neurodegeneration. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_4
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DOI: https://doi.org/10.1007/978-1-4939-2152-2_4
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Publisher Name: Humana Press, New York, NY
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