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Targeted Toxicants to Dopaminergic Neuronal Cell Death

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Book cover Neuronal Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1254))

Abstract

Parkinson’s disease (PD ) is mainly characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra resulting in chronic deficits in motor functions. Administration of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP ) produces PD symptoms and recapitulates the main features of PD in human and animal models . MPTP is converted to 1-methyl-4-phenylpyridine (MPP+ ), which is the active toxic compound that selectively destroys dopaminergic neurons. Here, we describe methods and protocols to evaluate MPTP/MPP+-induced dopaminergic neurodegeneration in both murine primary mesencephalic cultures and animal models. The ability of MPTP/MPP+ to cause dopaminergic neuronal cell death is assessed by immunostaining of tyrosine hydroxylase (TH ).

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Abbreviations

BSA:

Bovine serum albumin

HBSS:

Hank’s balanced salt solution

IACUC:

Institutional Animal Care and Use Committee

MPP:

1-Methyl-4-phenylpyridine

MPTP:

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine

OCT:

Optimum cutting temperature

PBS:

Phosphate-buffered saline

PD:

Parkinson’s disease

PDL:

Poly-d-lysine

PFA:

Paraformaldehyde

SNc:

Substantia nigra pars compacta

TH:

Tyrosine hydroxylase

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Acknowledgement

This work was supported by the National Institutes of Health grants, NS65167 and NS078237 to AK, and ES10586 and NS074443 to AGK. W. Eugene and Linda Lloyd Endowed Chair to AGK is also acknowledged.

Author information

Authors and Affiliations

  1. Parkinson’s Disorder Research Laboratory, Department of Biomedical Sciences, IA Center for Advanced Neurotoxicology, Iowa State University, 1600 S. 16th Street, Ames, IA, 50011, USA

    Huajun Jin, Arthi Kanthasamy, Dilshan S. Harischandra, Vellareddy Anantharam & Anumantha Kanthasamy

  2. Department of Molecular Pharmacology and Therapeutics, Loyola University Chicago, Maywood, IL, USA

    Ajay Rana

  3. Hines Veterans Affairs Medical Center, Hines, IL, USA

    Ajay Rana

Authors
  1. Huajun Jin
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  2. Arthi Kanthasamy
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  3. Dilshan S. Harischandra
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  4. Vellareddy Anantharam
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  5. Ajay Rana
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  6. Anumantha Kanthasamy
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Corresponding author

Correspondence to Anumantha Kanthasamy .

Editor information

Editors and Affiliations

  1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Laura Lossi

  2. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Adalberto Merighi

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Jin, H., Kanthasamy, A., Harischandra, D.S., Anantharam, V., Rana, A., Kanthasamy, A. (2015). Targeted Toxicants to Dopaminergic Neuronal Cell Death. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-2152-2_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2151-5

  • Online ISBN: 978-1-4939-2152-2

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