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Neuronal Cell Death pp 227–237Cite as

Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1254))

Abstract

Retinal cell apoptosis occurs in many eye conditions, including glaucoma , diabetic retinopathy and Alzheimer’s disease. Real-time detection of retinal cell apoptosis has potential clinical value in early disease detection, as well as evaluating disease progression and treatment efficacy. Here, we describe our novel imaging technology DARC (Detection of Apoptosing Retinal Cells ), which can be used to visualize single retinal neurons undergoing apoptosis in real time, by using fluorescently labeled Annexin A5 and confocal scanning laser ophthalmoscopy (cSLO ). Clinical trials of DARC in glaucoma patients are due to start shortly, but in this chapter, we describe this technique in experimental animal models.

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Conflict of Interest

M. Francesca Cordeiro is an inventor on patent applications owned by UCL and pertaining to Detection of Apoptosing Retinal Cells.

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Authors and Affiliations

  1. Glaucoma & Retinal Degeneration Research Group, Visual Neurosciences, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK

    Eduardo M. Normando, Mohammad H. Dehabadi, Li Guo, Lisa A. Turner, Gaia Pollorsi & M. Francesca Cordeiro

  2. The Western Eye Hospital, Imperial College Healthcare Trust, London, UK

    Eduardo M. Normando & M. Francesca Cordeiro

Authors
  1. Eduardo M. Normando
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  2. Mohammad H. Dehabadi
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  3. Li Guo
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  4. Lisa A. Turner
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  5. Gaia Pollorsi
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  6. M. Francesca Cordeiro
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Corresponding author

Correspondence to M. Francesca Cordeiro .

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Editors and Affiliations

  1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Laura Lossi

  2. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Adalberto Merighi

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Normando, E.M., Dehabadi, M.H., Guo, L., Turner, L.A., Pollorsi, G., Cordeiro, M.F. (2015). Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-2152-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2151-5

  • Online ISBN: 978-1-4939-2152-2

  • eBook Packages: Springer Protocols

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