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In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death

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Neuronal Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1254))

Abstract

Oxygen-glucose deprivation (OGD ) is widely used as an in vitro model for stroke , showing similarities with the in vivo models of brain ischemia . In order to perform OGD, cell or tissue cultures , such as primary neurons or organotypic slices , and acutely prepared tissue slices are usually incubated in a glucose-free medium under a deoxygenated atmosphere , for example in a hypoxic chamber . Here, we describe the step-by-step procedure to expose cultures and acute slices to OGD, focusing on the most suitable methods for assessing cellular death and/or viability . OGD is a simple yet highly useful technique, not only for the elucidation of the role of key cellular and molecular mechanisms underlying brain ischemia, but also for the development of novel neuroprotective strategies.

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Acknowledgements

The authors would like to thank Dr. U.F.J. Mayer for his invaluable assistance with the manuscript.

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário - Trindade 88040-900, Florianópolis, SC, Brazil

    Carla I. Tasca & Tharine Dal-Cim

  2. Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário - Trindade 88040-900, Florianópolis, SC, Brazil

    Helena Cimarosti

Authors
  1. Carla I. Tasca
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  2. Tharine Dal-Cim
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  3. Helena Cimarosti
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Corresponding author

Correspondence to Helena Cimarosti .

Editor information

Editors and Affiliations

  1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Laura Lossi

  2. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Adalberto Merighi

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Tasca, C.I., Dal-Cim, T., Cimarosti, H. (2015). In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-2152-2_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2151-5

  • Online ISBN: 978-1-4939-2152-2

  • eBook Packages: Springer Protocols

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