Abstract
Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.
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Rurup, W.F., Cornelissen, J.J.L.M., Koay, M.S.T. (2015). Recombinant Expression and Purification of “Virus-like” Bacterial Encapsulin Protein Cages. In: Orner, B. (eds) Protein Cages. Methods in Molecular Biology, vol 1252. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2131-7_6
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DOI: https://doi.org/10.1007/978-1-4939-2131-7_6
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