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Ferritin Encapsulation and Templated Synthesis of Inorganic Nanoparticles

  • Katherine W. Pulsipher
  • Ivan J. DmochowskiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1252)

Abstract

Understanding how inorganic nanoparticles interact with proteins is paramount to their safe and effective use in vivo. Ordered protein-inorganic nanomaterial assemblies will also enable the creation of patterned structures with useful physical properties. Thermophilic ferritin (tF) from Archaeoglobus fulgidus has unique structural features and self-assembly properties that facilitate stable but also reversible interaction with gold nanoparticles (AuNPs). In this chapter we describe how to express and purify tF and induce its assembly around AuNPs. We also describe methods for characterizing the tF-AuNP complex as well as templating NP growth within the protein cavity.

Key words

Ferritin assembly Protein self-assembly Nanoparticle-protein interaction Biomineralization Gold nanoparticle Ferritin Nano-bio interface 

Notes

Acknowledgments

The authors thank Eric Johnson for providing the Archaeoglobus fulgidus ferritin gene in the pAF0834 plasmid and Jeffery Saven for use of instruments. We also thank Joe Swift and Jasmina Cheung-Lau for doing the foundational work of this project. This work was supported by NSF CHE 0548188 and DMR-0520020.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of PennsylvaniaPhiladelphiaUSA

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