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Use of Protein Cages as a Template for Confined Synthesis of Inorganic and Organic Nanoparticles

  • Masaki Uchida
  • Shefah Qazi
  • Ethan Edwards
  • Trevor DouglasEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1252)

Abstract

Protein cages are hollow spherical proteins assembled from a defined number of subunits. Because they are extremely homogeneous in size and structure, their interior cavities can serve as ideal templates to encapsulate and synthesize well-defined nanoparticles. Here, we describe the exemplary synthesis of a hard and a soft material in two representative protein cages, i.e., magnetite nanoparticles in ferritin and a poly(2-aminoethyl)methacrylate inside a viral capsid derived from the bacteriophage P22.

Key words

Protein cages Ferritin Viral capsid Biomineralization Atom transfer radical polymerization (ATRP) 

Notes

Acknowledgment

This work was supported with grants from the US Department of Energy, Office of Basic Energy Sciences, Division of Materials Science and Engineering DE-FG02-07ER46477 (for the inorganic nanoparticle work) and the National Institutes of Health NIAID R01AI104905 (for the polymer work).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Masaki Uchida
    • 2
  • Shefah Qazi
    • 1
  • Ethan Edwards
    • 1
  • Trevor Douglas
    • 2
    Email author
  1. 1.Department of Chemistry and Biochemistry, Center for Bio-Inspired NanomaterialsMontana State UniversityBozemanUSA
  2. 2.Department of ChemistryIndiana UniversityBloomingtonUSA

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