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Protein Cages pp 101-113 | Cite as

Differential Scanning Calorimetry to Quantify the Stability of Protein Cages

  • Yu ZhangEmail author
  • Maziar S. Ardejani
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1252)

Abstract

Differential scanning calorimetry (DSC) is an experimental technique through which the differences in amount of heat required to maintain equal temperature between a sample and a reference cell are measured at various temperatures. The quantified heat relates to the differences in apparent heat capacity of the analytes. The data from DSC studies will thereby provide direct information about the energetics of thermally induced processes in the sample. Here we present a detailed protocol to quantify the thermostability of protein cage, bacterioferritin (BFR), using differential scanning calorimetry.

Key words

Protein cages Ferritin Thermostability Differential scanning calorimetry Thermodynamics Melting temperature Heat capacity 

Notes

Acknowledgment

This work was supported by grant from National Natural Science Foundation of China (No. 31200564) and Startup grant from Nanjing Forestry University (YJ2012-05) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.College of Chemical EngineeringNanjing Forestry UniversityNanjingChina
  2. 2.Department of ChemistryKing’s College LondonLondonUK

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