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Encapsulation of Nanoparticles in Virus Protein Shells

  • Irina B. Tsvetkova
  • Bogdan G. DragneaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1252)

Abstract

The self-assembly of virus-like particles may lead to materials which combine the unique characteristics of viruses, such as precise size control and responsivity to environmental cues, with the properties of abiotic cargo. For a few different viruses, shell proteins are amenable to the in vitro encapsulation of non-genomic cargo in a regular protein cage. In this chapter we describe protocols of high-efficiency in vitro self-assembly around functionalized gold nanoparticles for three examples of icosahedral and non-icosahedral viral protein cages derived from a plant virus, an animal virus, and a human retrovirus. These protocols can be readily adapted with small modifications to work for a broad variety of inorganic and organic nanoparticles.

Key words

Virus-like particle Gold nanoparticle Templated self-assembly Protein cage 

Notes

Acknowledgments

This work was supported by the US Department of Energy, Office of Science, Basic Energy Sciences, under Award # DE-SC0010507.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of ChemistryIndiana UniversityBloomingtonUSA

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