Abstract
Embryonic motoneurons depend on extracellular matrix (ECM), and molecules within which are potent mediators of survival, axonal growth, and guidance, exhibiting either attractive or repellent functions. Therefore, isolated embryonic spinal cord motoneurons were cultured on a three-dimensional substrate composed of different ECM molecules produced by different glia cell lines. The motoneuron-matrix co-culture could be used to analyze the modifying effects of matrix components like chondroitin sulfate proteoglycans, bifunctional molecules with modifying properties either due to their protein backbone or the glycan portion subdividing their action into protein and the glycan parts.
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We thank the JOVE journal for the kind permission regarding pictures and reproduction. This work was supported by the GRK 736 “Development and Plasticity of the Nervous System.”
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Conrad, R., Klausmeyer, A., Tsai, T., Faissner, A., Wiese, S. (2015). Extracellular Matrix Components as a Substrate for Outgrowing Motoneurons. In: Leach, J., Powell, E. (eds) Extracellular Matrix. Neuromethods, vol 93. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2083-9_7
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DOI: https://doi.org/10.1007/978-1-4939-2083-9_7
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