Abstract
Neurite outgrowth measurements often require time-consuming measurements of multiple images. The numerous experimental conditions lead to long analysis time and effort. A high-throughput assay provides a quick method to obtain quantitative measures of neurite outgrowth. In this chapter, the experimental setup and imaging protocol are described to efficiently process images of primary neurons or cell lines in order to quantitate parameters.
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References
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Beller, J.A., Hering, T.M., Snow, D.M. (2015). High-Throughput Quantitative Assay for Analyzing Neurite Outgrowth on a Uniform Substratum: The Cell-Substratum Assay. In: Leach, J., Powell, E. (eds) Extracellular Matrix. Neuromethods, vol 93. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2083-9_6
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DOI: https://doi.org/10.1007/978-1-4939-2083-9_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2082-2
Online ISBN: 978-1-4939-2083-9
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