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Evaluating the Spatial and Temporal Protein Production in Neural Tissue Engineering Constructs In Vitro

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Extracellular Matrix

Part of the book series: Neuromethods ((NM,volume 93))

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Abstract

Tissue engineering constructs are uniquely positioned not only for in vivo regenerative therapeutic applications but also to evaluate cellular responses to modifications in finely tuned three-dimensional (3-D) microenvironments as opposed to traditional two-dimensional (2-D) culture systems. However, moving into a 3-D system presents complications of assessing spatial and temporal alterations in cellular behavior (e.g., protein synthesis, motility, etc.). Here, we describe methods to evaluate spatial and temporal protein production in neural tissue engineering construct.

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Author information

Authors and Affiliations

  1. School of Biological and Health Systems Engineering, Ira A. Fulton Schools of Engineering, Arizona State University, 879709, Tempe, AZ, 85287-9709, USA

    Caroline P. Addington, Christine Pauken & Sarah E. Stabenfeldt Ph.D.

Authors
  1. Caroline P. Addington
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  2. Christine Pauken
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  3. Sarah E. Stabenfeldt Ph.D.
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Corresponding author

Correspondence to Sarah E. Stabenfeldt Ph.D. .

Editor information

Editors and Affiliations

  1. University of Maryland, Baltimore County, Baltimore, Maryland, USA

    Jennie B. Leach

  2. Department of Anatomy & Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland, USA

    Elizabeth M. Powell

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© 2015 Springer Science+Business Media New York

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Addington, C.P., Pauken, C., Stabenfeldt, S.E. (2015). Evaluating the Spatial and Temporal Protein Production in Neural Tissue Engineering Constructs In Vitro. In: Leach, J., Powell, E. (eds) Extracellular Matrix. Neuromethods, vol 93. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2083-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2083-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2082-2

  • Online ISBN: 978-1-4939-2083-9

  • eBook Packages: Springer Protocols

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