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Practical Structured Illumination Microscopy

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Advanced Fluorescence Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1251))

Abstract

Structured illumination microscopy (SIM) is a method that can double the spatial resolution of wide-field fluorescence microscopy in three dimensions by using spatially structured illumination light. In this chapter, we introduce the basic principles of SIM and describe in detail several different implementations based on either a diffraction grating or liquid crystal spatial light modulators. We also describe nonlinear SIM, a method that in theory can achieve unlimited resolution. In addition, we discuss a number of key points important for high-resolution imaging.

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References

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Author information

Authors and Affiliations

  1. Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, 02115, USA

    E. Hesper Rego

  2. Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, 20147, USA

    Lin Shao

Authors
  1. E. Hesper Rego
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  2. Lin Shao
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Corresponding author

Correspondence to Lin Shao .

Editor information

Editors and Affiliations

  1. Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Peter J. Verveer

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© 2015 Springer Science+Business Media New York

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Rego, E.H., Shao, L. (2015). Practical Structured Illumination Microscopy. In: Verveer, P. (eds) Advanced Fluorescence Microscopy. Methods in Molecular Biology, vol 1251. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2080-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-2080-8_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2079-2

  • Online ISBN: 978-1-4939-2080-8

  • eBook Packages: Springer Protocols

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