Abstract
Human skin-derived precursor cells are a multipotent stem cell population that resides within the dermis throughout adulthood. Human skin-derived precursor cells can be isolated, purified, and expanded in large quantities from any patient, in health and disease, and differentiated to mesodermal and ectodermal cell types. Recently, it was also found that they can be directed towards hepatic cells with acquired properties of toxicological relevance. As such, they represent a valuable cell source for the further development of human-relevant in vitro models for the identification and quantification of hepatotoxic compounds. In this chapter, a robust basic methodology to isolate, expand, and differentiate human skin-derived precursor cells into hepatic cells in a sequential and time-dependent way is provided.
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References
Rodrigues RM, De Kock J, Branson S et al (2014) Human skin-derived stem cells as a novel cell source for in vitro hepatotoxicity screening of pharmaceuticals. Stem Cells Dev 23:44–55
Szkolnicka D, Farnworth SL, Lucendo-Villarin B et al (2014) Accurate prediction of drug-induced liver injury using stem cell-derived populations. Stem Cells Transl Med 3:141–148
Toma JG, McKenzie IA, Bagli D et al (2005) Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells 23:727–737
Suflita MT, Pfaltzgraff ER, Mundell NA et al (2013) Ground-state transcriptional requirements for skin-derived precursors. Stem Cells Dev 22:1779–1788
Gago N, Perez-Lopez V, Sanz-Jaka JP et al (2009) Age-dependent depletion of human skin-derived progenitor cells. Stem Cells 27:1164–1172
Buranasinsup S, Sila-Asna M, Bunyaratvej N et al (2006) In vitro osteogenesis from human skin-derived precursor cells. Dev Growth Differ 48:263–269
De Kock J, Najar M, Bolleyn J et al (2012) Mesoderm-derived stem cells: the link between the transcriptome and their differentiation potential. Stem Cells Dev 21:3309–3323
Hunt DPJ, Jahoda C, Chandran S (2009) Multipotent skin-derived precursors: from biology to clinical translation. Curr Opin Biotechnol 20:522–530
Shih DTB, Lee DC, Chen SC et al (2005) Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue. Stem Cells 23:1012–1020
McKenzie IA, Biernaskie J, Toma JG et al (2006) Skin-derived precursors generate myelinating Schwann cells for the injured and dysmyelinated nervous system. J Neurosci 26:6651–6660
Biernaskie J, Sparling JS, Liu J et al (2007) Skin-derived precursors generate myelinating Schwann cells that promote remyelination and functional recovery after contusion spinal cord injury. J Neurosci 27:9545–9559
Fernandes KJL, Kobayashi NR, Gallagher CJ et al (2006) Analysis of the neurogenic potential of multipotent skin-derived precursors. Exp Neurol 201:32–48
Lavoie JF, Biernaskie JA, Chen Y et al (2009) Skin-derived precursors differentiate into skeletogenic cell types and contribute to bone repair. Stem Cells Dev 18:893–906
De Kock J, Snykers S, Ramboer E et al (2011) Evaluation of the multipotent character of human foreskin-derived precursor cells. Toxicol In Vitro 25:1191–1202
Steinbach SK, El-Mounayri O, DaCosta RS et al (2011) Directed differentiation of skin-derived precursors into functional vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 31:2938–2948
De Kock J, Vanhaecke T, Biernaskie J et al (2009) Characterization and hepatic differentiation of skin-derived precursors from adult foreskin by sequential exposure to hepatogenic cytokines and growth factors reflecting liver development. Toxicol In Vitro 23:1522–1527
Acknowledgements
This work has received funding from Grants of the Fund for Scientific Research in Flanders (FWO-Vlaanderen), the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen), the Research Council (OZR) of the Vrije Universiteit Brussel, Wetenschappelijk Fonds Willy Gepts from the University Hospital of the Vrije Universiteit Brussel (UZ Brussel) and from BRUSTEM-2, an impulse programme of the Institute for the encouragement of Scientific Research and Innovation of Brussels (INNOVIRIS), HEPRO-2, an Interuniversity Attraction Pole programme of the Belgian Science Policy Office (BELSPO), the European Community’s Seventh Framework Programme (FP7/2007–2013) under grant agreement No. 20161 (ESNATS), No. 266838 (DETECTIVE), and No. 266777 (HEMIBIO).
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De Kock, J., Rodrigues, R.M., Buyl, K., Vanhaecke, T., Rogiers, V. (2015). Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation. In: Vinken, M., Rogiers, V. (eds) Protocols in In Vitro Hepatocyte Research. Methods in Molecular Biology, vol 1250. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2074-7_8
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DOI: https://doi.org/10.1007/978-1-4939-2074-7_8
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