Abstract
The identification of cancer cell-specific ligands is a key requirement for the targeted delivery of chemotherapeutic agents. Usually phage display system is employed to discover cancer-specific peptides through a biopanning process. Synthetic peptide array libraries can be used as a complementary method to phage display for screening and identifying cancer cell-specific ligands. Here, we describe a peptide array-whole cell binding assay to identify cancer cell-specific peptides. A peptide array library based on a lead dodecapeptide, p160, is synthesized on a functionalized cellulose membrane using solid phase chemistry and a robotic synthesizer. The relative binding affinity of the peptide library is evaluated by incubating the library with fluorescently labeled cancerous or non-cancerous cells. Thereby the assay allows picking peptides that show selective and high binding to cancerous cells. These peptides represent potential candidates for use in cancer-targeted drug delivery, imaging, and diagnosis.
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Acknowledgments
This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC). The infrastructure support from the Canada Foundation for Innovation (CFI) is also acknowledged.
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Kaur, K., Ahmed, S., Soudy, R., Azmi, S. (2015). Screening Peptide Array Library for the Identification of Cancer Cell-Binding Peptides. In: Derda, R. (eds) Peptide Libraries. Methods in Molecular Biology, vol 1248. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2020-4_16
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DOI: https://doi.org/10.1007/978-1-4939-2020-4_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2019-8
Online ISBN: 978-1-4939-2020-4
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