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MeDIP-on-Chip for Methylation Profiling

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Cervical Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1249))

Abstract

DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlations with biological events or disease progression. Methylated DNA immunoprecipitation (MeDIP) uses an antibody that efficiently enriches methylated DNA fragments for downstream locus-specific or genome-wide analyses. MeDIP-on-Chip uses the MeDIP approach in combination with a tiling array for the investigation of genome-wide DNA methylation patterns (or DNA methylomics). The following protocol describes the application of MeDIP to the hybridization of DNA microarrays and data analysis.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Tri-Service General Hospital and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei City, Taiwan, ROC

    Yaw-Wen Hsu

  2. Department of Obstetrics and Gynecology, Shuang Ho Hospital, Taipei Medical University, No. 291, Jhongjheng Rd., Jhonghe, New Taipei City, 23561, Taiwan, (ROC)

    Rui-Lan Huang

  3. School of Medicine; Department of Obstetrics and Gynecology, Shuang Ho Hospital, Taipei Medical University, No. 291, Jhongjheng Rd., Jhonghe, New Taipei City, 23561, Taiwan, (ROC)

    Hung-Cheng Lai M.D., Ph.D.

Authors
  1. Yaw-Wen Hsu
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  2. Rui-Lan Huang
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  3. Hung-Cheng Lai M.D., Ph.D.
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Corresponding author

Correspondence to Hung-Cheng Lai M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Touro University California, Vallejo, California, USA

    Daniel Keppler

  2. Touro University California, Vallejo, California, USA

    Athena W. Lin

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© 2015 Springer Science+Business Media New York

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Hsu, YW., Huang, RL., Lai, HC. (2015). MeDIP-on-Chip for Methylation Profiling. In: Keppler, D., Lin, A. (eds) Cervical Cancer. Methods in Molecular Biology, vol 1249. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2013-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-2013-6_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2012-9

  • Online ISBN: 978-1-4939-2013-6

  • eBook Packages: Springer Protocols

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