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Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies pp 513–527Cite as

Proteomics Characterization of Tick-Host-Pathogen Interactions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1247))

Abstract

Ticks are blood-feeding arthropod ectoparasites of wild and domestic animals that transmit disease-causing pathogens to humans and animals worldwide and a good model for the characterization of tick-host-pathogen interactions. Tick-host-pathogen interactions consist of dynamic processes involving genetic traits of hosts, pathogens, and ticks that mediate their development and survival. Proteomics provides information on the protein content of cells and tissues that may differ from results at the transcriptomics level and may be relevant for basic biological studies and vaccine antigen discovery. In this chapter, we describe various methods for protein extraction and for proteomics analysis in ticks based on one-dimensional gel electrophoresis to characterize tick-host-pathogen interactions. Particularly relevant for this characterization is the use of blood-fed ticks. Therefore, we put special emphasis on working with replete ticks collected after feeding on vertebrate hosts.

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Acknowledgments

We thank the CMBSO proteomics facility (Centro de Biología Molecular Severo Ochoa, Madrid, Spain) for technical assistance. This research was supported by grants BFU2011-23896 and the EU FP7 ANTIGONE project number 278976. M. Popara is an early-stage researcher supported by the POSTICK ITN (postgraduate training network for capacity building to control ticks and tick-borne diseases) within the FP7-PEOPLE—ITN program (EU Grant No. 238511).

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Authors and Affiliations

  1. SaBio. Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005, Ciudad Real, Spain

    Marina Popara, Margarita Villar & José de la Fuente

  2. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    José de la Fuente

Authors
  1. Marina Popara
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  2. Margarita Villar
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  3. José de la Fuente
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Corresponding author

Correspondence to José de la Fuente .

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Editors and Affiliations

  1. Instituto Nacional de Investigação Agrária e Veterinária, IP and Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal

    Mónica V. Cunha

  2. Instituto Nacional de Investigação Agrária e Veterinária, IP, Lisboa, Portugal and School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, United Kingdom

    João Inácio

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Popara, M., Villar, M., de la Fuente, J. (2015). Proteomics Characterization of Tick-Host-Pathogen Interactions. In: Cunha, M., Inácio, J. (eds) Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Methods in Molecular Biology, vol 1247. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2004-4_34

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  • DOI: https://doi.org/10.1007/978-1-4939-2004-4_34

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2003-7

  • Online ISBN: 978-1-4939-2004-4

  • eBook Packages: Springer Protocols

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