Abstract
Digital gene expression (DGE) analysis is a cost-effective method for large-scale quantitative transcriptome analysis using second generation sequencing. Here we describe how adaptation of DGE with barcode indexing in large segregating plant populations of over 100 genotypes can be applied for successful expression QTL (eQTL) and gene expression network analysis to develop transcript-based markers for breeding.
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Acknowledgements
The development of this protocol for high-throughput mapping in large plant populations was funded by the Deutsche Forschungsgemeinschaft (DFG) grants SN14/7-2, SN14/11-1 and WA 2161/2-1.The authors thank Bashir Hosseini, Anja Pöltl, and Liane Renno for technical assistance.
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Obermeier, C., Salazar-Colqui, B.M., Spamer, V., Snowdon, R. (2015). Multiplexed Digital Gene Expression Analysis for Genetical Genomics in Large Plant Populations. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_9
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DOI: https://doi.org/10.1007/978-1-4939-1966-6_9
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