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Screening of Mutations by TILLING in Plants

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Plant Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1245))

Abstract

TILLING (Targeting Induced Local Lesions IN Genomes) is a well-known reverse genetics technique designed to detect unknown SNPs (single nucleotide polymorphisms) in genes of interest using an enzymatic digestion and is widely employed in plant and animal genomics. The main advantage of this technique is that it allows for the high-throughput identification of an allelic series of mutants with a range of modified functions for a particular gene. In this chapter, we aim to give a detailed introduction of how to establish a TILLING platform for identifying mutants in plants, including generation of a large mutant population, DNA and seed library preparation, mutation identification based on a LI-COR4300 DNA analyzer, and confirmation of functions of the mutated genes.

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Author information

Authors and Affiliations

  1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China

    Nian Wang

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, No. 1 Shizishan Street, Wuhan, 430070, China

    Lei Shi

Authors
  1. Nian Wang
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  2. Lei Shi
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Corresponding author

Correspondence to Lei Shi .

Editor information

Editors and Affiliations

  1. School of Plant Biology, The University of Western Australia, Crawley, West Australia, Australia

    Jacqueline Batley

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Wang, N., Shi, L. (2015). Screening of Mutations by TILLING in Plants. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-1966-6_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1965-9

  • Online ISBN: 978-1-4939-1966-6

  • eBook Packages: Springer Protocols

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