Abstract
The unambiguous differentiation of crop genotypes is often laborious or expensive. A rapid, robust, and cost-efficient marker system is required for routine genotyping in plant breeding and marker-assisted selection. We describe the Inter-SINE Amplified Polymorphism (ISAP) system that is based on standard molecular methods resulting in genotype-specific fingerprints at high resolution. These markers are derived from Short Interspersed Nuclear Elements (SINEs) which are dispersed repetitive sequences present in most if not all plant genomes and can be efficiently extracted from plant genome sequences. The ISAP method was developed on potato as model plant but is also transferable to other plant species.
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Acknowledgement
We gratefully acknowledge the funding of the KMU-Innovativ-project (grant no. 0315425) by the German Federal Ministry of Education and Research.
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Wenke, T., Seibt, K.M., Döbel, T., Muders, K., Schmidt, T. (2015). Inter-SINE Amplified Polymorphism (ISAP) for Rapid and Robust Plant Genotyping. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_14
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DOI: https://doi.org/10.1007/978-1-4939-1966-6_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1965-9
Online ISBN: 978-1-4939-1966-6
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