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Live Cell Imaging of the Cytoskeleton and Cell Wall Enzymes in Plant Cells

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Plant Cell Expansion

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1242))

Abstract

The use of live imaging techniques to visualize the dynamic changes and interactions within plant cells has given us detailed information on the function and organization of the cytoskeleton and cell wall associated proteins. This information has grown with the constant improvement in imaging hardware and molecular tools. In this chapter, we describe the procedure for the preparation and live visualization of fluorescent protein fusions associated with the cytoskeleton and the cell wall in Arabidopsis.

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Acknowledgements

We would like to thank Dr. Staffan Persson at the Max Planck Institute of Molecular Plant Physiology for his comments on the chapter.

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Correspondence to Arun Sampathkumar .

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Sampathkumar, A., Wightman, R. (2015). Live Cell Imaging of the Cytoskeleton and Cell Wall Enzymes in Plant Cells. In: Estevez, J. (eds) Plant Cell Expansion. Methods in Molecular Biology, vol 1242. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1902-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1902-4_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1901-7

  • Online ISBN: 978-1-4939-1902-4

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