Abstract
Cryopreservation is an essential technique to preserve stem cells, semipermanently sustaining their potentials. There are two main approaches of cryopreservation for human pluripotent stem cells (hPSCs). The first is the vitrification, which involves instantaneous freeze and thaw of hPSCs. The second is the conventional slow-cooling method and a rapid thaw. Both cryopreservation protocols have been standardized and optimized to yield high survivability of hPSCs.
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References
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Acknowledgements
This work was supported by New Energy and Industrial Technology Development Organization (NEDO), Japan; Ministry of Health, Labour and Welfare, Japan.
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© 2015 Springer Science+Business Media New York
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Miyazaki, T., Suemori, H. (2015). Cryopreservation of Human Pluripotent Stem Cells: A General Protocol. In: Rich, I. (eds) Stem Cell Protocols. Methods in Molecular Biology, vol 1235. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1785-3_9
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DOI: https://doi.org/10.1007/978-1-4939-1785-3_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1784-6
Online ISBN: 978-1-4939-1785-3
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