Abstract
The bone marrow (BM) is permeated with sinusoidal vessels lined with sinusoidal endothelial cells (SEC), which are crucial for BM physiology and hemopoietic stem cell (HSC) renewal. However, little is known about the characteristics or functional capacity of bone marrow sinusoidal endothelial cells (BMSEC). One significant barrier to the study of BMSEC is the lack of specific cell surface markers that can be used to isolate these cells. Nevertheless, BMSEC possess one exceptional property, namely, the ability to scavenge large amounts of soluble waste molecules such as advanced glycation end-products (AGE) and we have utilized this to label BMSEC for cell sorting and isolation. We describe the means to produce and fluorescently label AGE, its use as a functional in vivo marker of BMSEC and the isolation of these cells from murine BM using fluorescent activated cell separation (FACS).
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Acknowledgements
P.M. is supported by a grant from the Tromso Research Foundation/Trond Mohn and from the Northern Norway Regional Health Authority Post-Doctoral Fellowship (HelseNord, SFP1000-11 ID5432). AO has a Northern Norway Regional Health Authority Post-Doctoral Fellowship (HelseNord, SFP1000-11 ID5432). S.N. has an ARC Future Fellowship and a Science and Industry Endowment Fund (SIEF) project grant. The authors would like to thank Brenda Williams and Shen Heazlewood for critically reviewing the chapter.
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McCourt, P.A.G., Oteiza, A., Cao, B., Nilsson, S.K. (2015). Isolation of Murine Bone Marrow Scavenging Sinusoidal Endothelial Cells. In: Rich, I. (eds) Stem Cell Protocols. Methods in Molecular Biology, vol 1235. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1785-3_6
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DOI: https://doi.org/10.1007/978-1-4939-1785-3_6
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Publisher Name: Humana Press, New York, NY
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