Sepsis pp 91-96 | Cite as

Detection of Carbapenemases Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay

  • Jaroslav HrabákEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1237)


Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently introduced to many diagnostic microbiological laboratories. Besides the identification of bacteria and fungi, that technique provides a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by β-lactamases. Here, we describe an assay allowing a detection of meropenem hydrolysis in clinical isolates of Enterobacteriaceae, Pseudomonas spp., and Acinetobacter baumannii using MALDI-TOF MS. This method is able to confirm carbapenemases within 3 h. The results are important for proper and fast intervention to limit the spread of carbapenemase-producing bacteria and provide information for appropriate initial therapy of the infections caused by these microbes.


Sodium Salt Acinetobacter Baumannii Dihydroxybenzoic Acid Suspension Buffer Chromogenic Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the grant Nr. NT11032-6/2010 and by the Charles University Research Fund (Nr. P36).


  1. 1.
    Hrabak J, Chudackova E, Walkova R (2013) Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clin Microbiol Rev 26:103–114PubMedCrossRefPubMedCentralGoogle Scholar
  2. 2.
    Hrabak J, Walkova R, Studentova V, Chudackova E, Bergerova T (2011) Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 49: 3222–3227PubMedCrossRefPubMedCentralGoogle Scholar
  3. 3.
    Burckhardt I, Zimmermann S (2011) Using matrix-assisted laser desorption ionization–time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours. J Clin Microbiol 49:3321–3324PubMedCrossRefPubMedCentralGoogle Scholar
  4. 4.
    Hrabak J, Studentova V, Walkova R, Zemlickova H, Jakubu V, Chudackova E, Gniadkowski M, Pfeifer Y, Perry JD, Wilkinson K, Bergerová T (2012) Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:2441–2443PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of MicrobiologyFaculty of Medicine and University Hospital in Plzen, Charles University in PraguePlzenCzech Republic

Personalised recommendations