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Sepsis pp 73-90 | Cite as

Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-Based Approach

  • Teresa Raich
  • Scott PowellEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1237)

Abstract

Rapid identification and characterization of bacterial and fungal pathogens present in the bloodstream are essential for optimal patient management and are associated with improved patient outcomes, improved antimicrobial stewardship, improved infection control, and reduced healthcare costs. Microarrays serve as reliable platforms for the identification of these bloodstream pathogens and their associated antimicrobial resistance genes, if present. Nanosphere’s (Nanosphere, Inc., Northbrook, IL, USA) Verigene Gram-Positive Blood Culture Nucleic-Acid Test (BC-GP) is one such microarray-based approach for the detection of bacteria that cause bloodstream infection. Here, we describe the design of the microarray-based Verigene BC-GP Test, the steps necessary for performing the test, and the different components of the test including nucleic acid extraction and hybridization of target nucleic acid to a microarray.

Key words

Bloodstream infection Microarray Verigene BC-GP Oligonucleotide Extraction Hybridization Sample-to-result 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Nanosphere Inc.NorthbrookUSA

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