Sepsis pp 35-46 | Cite as

Technical Improvements in Culturing Blood

  • Giacomo PardiniEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1237)


Blood culture is a laboratory test where a blood specimen, taken from a patient, is inoculated into bottles containing culture media to determine if infection-causing microorganisms (bacteria or fungi) have invaded the patient’s bloodstream. This test is an important investigation with major implications for the diagnosis and treatment of patients with bloodstream infections and possible sepsis. Moreover, blood culture will also provide the etiologic agent for antimicrobial susceptibility testing, enabling optimization of antibiotic therapy with significant impact on the outcome of the disease. Even if the potential benefices of blood culture are well known, critical factors mainly in pre- and post-analytical phases can reduce the clinical value of this test.

Key words

Bloodstream infection Blood culture Sepsis Culture media Sample collection Diagnostic tests Gram staining Preliminary tests 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Alifax S.p.APolveraraItaly

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