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Sepsis pp 129-138 | Cite as

Broad-Range PCR for the Identification of Bacterial and Fungal Pathogens from Blood: A Sequencing Approach

  • Eva Leitner
  • Harald H. KesslerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1237)

Abstract

Broad-range PCR has become a valuable tool for the identification of microorganisms in the clinical laboratory over the last years. It was primarily used to identify slow-growing and fastidious microorganisms with poor biochemical activity. Nowadays, it is also used to identify microorganisms directly from clinical samples such as blood or punctuates from primarily sterile body sites. In these specimens, the usage of broad-range PCR is challenging regarding contamination and standardization. To overcome these problems, a new test system, the SepsiTest™, was introduced recently employing broad-range PCR for the identification of microorganisms in septic patients. In this chapter, the test system is described and the equipment necessary listed.

Key words

Sepsis Broad-range PCR Sequencing SepsiTest Identification Bacteria Fungi 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institute of Hygiene, Microbiology and Environmental Medicine (IHME)Medical University of GrazGrazAustria

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