Sepsis pp 121-128 | Cite as

Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols

  • Michael G. LorenzEmail author
  • Claudia Disqué
  • Helge Mühl
Part of the Methods in Molecular Biology book series (MIMB, volume 1237)


Automation in DNA isolation is a necessity for routine practice employing molecular diagnosis of infectious agents. To this end, the development of automated systems for the molecular diagnosis of microorganisms directly in blood samples is at its beginning. Important characteristics of systems demanded for routine use include high recovery of microbial DNA, DNA-free containment for the reduction of DNA contamination from exogenous sources, DNA-free reagents and consumables, ideally a walkaway system, and economical pricing of the equipment and consumables. Such full automation of DNA extraction evaluated and in use for sepsis diagnostics is yet not available. Here, we present protocols for the semiautomated isolation of microbial DNA from blood culture and low- and high-volume blood samples. The protocols include a manual pretreatment step followed by automated extraction and purification of microbial DNA.

Key words

Microbial DNA extraction Blood culture Blood Human DNA removal MolYsisTM easyMAG® SelectNATM Bacteremia Fungemia 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Michael G. Lorenz
    • 1
    Email author
  • Claudia Disqué
    • 1
  • Helge Mühl
    • 1
  1. 1.Molzym GmbH & Co. KGBremenGermany

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