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Trafficking and Function of GPCRs in the Endosomal Compartment

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Book cover Nuclear G-Protein Coupled Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1234))

Abstract

New methods based on fluorescently labeled agonists, genetically encoded fluorescent sensors, and advanced microscopy techniques, such as fluorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular trafficking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.

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Author information

Authors and Affiliations

  1. Bio-Imaging Center/Rudolf Virchow Center for Experimental Biomedicine, and Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, 97078, Würzburg, Germany

    Davide Calebiro, Amod Godbole, Sandra Lyga & Martin J. Lohse

Authors
  1. Davide Calebiro
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  2. Amod Godbole
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  3. Sandra Lyga
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  4. Martin J. Lohse
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Corresponding author

Correspondence to Davide Calebiro .

Editor information

Editors and Affiliations

  1. Montreal Heart Institute & Université de Montréal, Montreal, Québec, Canada

    Bruce G. Allen

  2. Pharmacology and Therapeutics, McGill University, Montreal, Québec, Canada

    Terence E. Hébert

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© 2015 Springer Science+Business Media New York

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Calebiro, D., Godbole, A., Lyga, S., Lohse, M.J. (2015). Trafficking and Function of GPCRs in the Endosomal Compartment. In: Allen, B., Hébert, T. (eds) Nuclear G-Protein Coupled Receptors. Methods in Molecular Biology, vol 1234. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1755-6_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1755-6_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1754-9

  • Online ISBN: 978-1-4939-1755-6

  • eBook Packages: Springer Protocols

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