Abstract
Detergent-resistant membranes (DRMs) isolated from cells are enriched in proteins and lipids with a high affinity for lipid rafts, or membrane microdomains in the liquid-ordered phase. Enrichment in DRMs provides a good indication that a protein is “raftophilic,” and may be present in rafts in cell membranes before extraction. Here, I describe preparation of Triton X-100-insoluble DRMs from cultured mammalian cells on sucrose gradients. Methods for analyzing the distribution of particular proteins across the gradient, and for recovering DRMs for further use are presented.
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Acknowledgement
This work was supported by NIH grant GM47897 to DAB.
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Brown, D.A. (2015). Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells. In: Owen, D. (eds) Methods in Membrane Lipids. Methods in Molecular Biology, vol 1232. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1752-5_5
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DOI: https://doi.org/10.1007/978-1-4939-1752-5_5
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