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Methods in Membrane Lipids pp 273–290Cite as

The Laurdan Spectral Phasor Method to Explore Membrane Micro-heterogeneity and Lipid Domains in Live Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1232))

Abstract

In this method paper we describe the spectral phasor analysis applied to Laurdan emission for the assessment of the fluidity of different membranes in live cells. We first introduce the general context and then we show how to obtain the spectral phasor from data acquired using a commercial microscope.

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Acknowledgments

We thank Milka Stakic for cultivating the cells. We also want to thank Michelle A. Digman for helping with the GUV’s preparation, for the reagents for CT-B-A594 staining and valuable suggestions. We want to thank Susana A. Sanchez, Moshe Levi, and Luca Lanzano for important suggestions and help during cholesterol depletion experiments and William Rosales for his helpful assistance during GUV’s preparation. This work is supported in part by NIH-P41-RR03155, P41 GM103540 and NIH P50-GM076516.

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Authors and Affiliations

  1. Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, 3208 Natural Sciences II, Irvine, CA, 92697-2715, USA

    Ottavia Golfetto, Elizabeth Hinde & Enrico Gratton

  2. Faculty of Medicine, Centre for Vascular Research, University of New South Wales, Sydney, NSW, Australia

    Elizabeth Hinde

Authors
  1. Ottavia Golfetto
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  2. Elizabeth Hinde
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  3. Enrico Gratton
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Corresponding author

Correspondence to Enrico Gratton .

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Editors and Affiliations

  1. Department of Physics and Randall Division of Cell and Molecular Biophysics, King’s College London, London, United Kingdom

    Dylan M. Owen

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Golfetto, O., Hinde, E., Gratton, E. (2015). The Laurdan Spectral Phasor Method to Explore Membrane Micro-heterogeneity and Lipid Domains in Live Cells. In: Owen, D. (eds) Methods in Membrane Lipids. Methods in Molecular Biology, vol 1232. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1752-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-1752-5_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1751-8

  • Online ISBN: 978-1-4939-1752-5

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