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Analysis of the Heavy-Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices

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Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.

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References

  1. Milner CM, Higman VA, Day AJ (2006) TSG-6: a pluripotent inflammatory mediator? Biochem Soc Trans 34(Pt 3):446–450

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  4. Swaidani S, Cheng G, Lauer ME et al (2013) TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma. J Biol Chem 288(1):412–422

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Author information

Authors and Affiliations

  1. Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH, 44195, USA

    Mark E. Lauer, Jacqueline Loftis, Carol de la Motte & Vincent C. Hascall

Authors
  1. Mark E. Lauer
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  2. Jacqueline Loftis
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  3. Carol de la Motte
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  4. Vincent C. Hascall
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Corresponding author

Correspondence to Mark E. Lauer .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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© 2015 Springer Science+Business Media New York

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Lauer, M.E., Loftis, J., de la Motte, C., Hascall, V.C. (2015). Analysis of the Heavy-Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_42

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_42

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

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