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Cell Substrate Patterning with Glycosaminoglycans to Study Their Biological Roles in the Central Nervous System

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Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

Microcontact printing (μCP) based techniques have been developed for creating cell culture substrates with discrete placement of CNS-expressed molecules. These substrates can be used to study various components of the complex molecular environment in the central nervous system (CNS) and related cellular responses. Macromolecules such as glycosaminoglycans (GAGs), proteoglycans (PGs), or proteins are amenable to printing. Detailed protocols for both adsorption based as well as covalent reaction printing of cell culture substrates are provided. By utilizing a modified light microscope, precise placement of two or more types of macromolecules by sequential μCP can be used to create desired spatial arrangements containing multicomponent PG, GAG, and protein surface patterns for studying CNS cell behavior. Examples of GAG stripe assays for neuronal pathfinding and directed outgrowth, and dot gradients of PG + laminin for astrocyte migration studies are provided.

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Acknowledgment

This work was supported by NIH grant R01 NS57144.

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Authors and Affiliations

  1. Department of Bioengineering, University of Utah, 20 S. 2030 E. Rm 108, Salt Lake City, UT, 84112, USA

    Tony W. Hsiao, Vimal P. Swarup, Colin D. Eichinger & Vladimir Hlady

Authors
  1. Tony W. Hsiao
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  2. Vimal P. Swarup
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  3. Colin D. Eichinger
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  4. Vladimir Hlady
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Corresponding author

Correspondence to Vladimir Hlady .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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Hsiao, T.W., Swarup, V.P., Eichinger, C.D., Hlady, V. (2015). Cell Substrate Patterning with Glycosaminoglycans to Study Their Biological Roles in the Central Nervous System. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_35

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_35

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

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