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Mapping Proteoglycan Functions with Glycosidases

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Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

The intrinsic and extrinsic factors that contribute to stem and neuronal precursor cell maintenance and/or differentiation remain poorly understood. Proteoglycans, major residents of the stem cell microenvironment, modulate key signaling cues and are of particular importance. We have taken a loss-of-function approach, by developing a library of bacterial lyases and sulfatases to specifically remodel the ECM and test the functional role of glycosaminoglycans (GAGs) in cell self-renewal, maintenance, and differentiation.

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Acknowledgements

This work was supported by NIH grants HD-009402, HD 017332, HD 054275, HD 06976, and 10035 from the Mizutani Foundation for Glycoscience. We thank James Mensch for help in assembling and proofing this manuscript.

Author information

Authors and Affiliations

  1. Department of Pediatrics, University of Chicago, 5841S. Maryland Avenue, Chicago, IL, 60637, USA

    Mauricio Cortes, Leslie K. Cortes & Nancy B. Schwartz Ph.D.

  2. Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02115, USA

    Mauricio Cortes

  3. New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA

    Leslie K. Cortes

Authors
  1. Mauricio Cortes
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  2. Leslie K. Cortes
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  3. Nancy B. Schwartz Ph.D.
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Corresponding author

Correspondence to Nancy B. Schwartz Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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© 2015 Springer Science+Business Media New York

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Cortes, M., Cortes, L.K., Schwartz, N.B. (2015). Mapping Proteoglycan Functions with Glycosidases. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_34

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_34

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

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