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Glycosaminoglycans pp 129–141Cite as

Validated Capillary Electrophoretic Assays for Disaccharide Composition Analysis of Galactosaminoglycans in Biologic Samples and Drugs/Nutraceuticals

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

Capillary electrophoresis is a separation technique with high resolving power and sensitivity with applications in glycosaminoglycan analysis. In this chapter, we present validated protocols for determining the variously sulfated chondroitin or dermatan sulfate-derived disaccharides. These approaches involve degradation of the polysaccharides with specific chondro/dermato-lyases and electrophoretic analysis with capillary zone electrophoresis in a low pH operating buffer and reversed polarity. This methodology has been applied to drug/nutraceutical formulations or to biologic samples (blood serum, lens capsule) and has been validated. Analysis of biologic tissue samples is often more demanding in terms of detection sensitivity, and thus concentration pretreatment steps and/or a derivatization step with 2-aminoacridone are often advisable.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Department of Chemistry, University of Patras, 1414, 26504, Patras, Greece

    Athanasia P. Asimakopoulou, Christina Malavaki, Nikolaos A. Afratis, Achilleas D. Theocharis & Nikos K. Karamanos

  2. Department of Pharmacy, University of Patras, 1414, 26504, Patras, Greece

    Fotini N. Lamari

Authors
  1. Athanasia P. Asimakopoulou
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  2. Christina Malavaki
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  3. Nikolaos A. Afratis
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  4. Achilleas D. Theocharis
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  5. Fotini N. Lamari
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  6. Nikos K. Karamanos
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Corresponding author

Correspondence to Nikos K. Karamanos .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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Asimakopoulou, A.P., Malavaki, C., Afratis, N.A., Theocharis, A.D., Lamari, F.N., Karamanos, N.K. (2015). Validated Capillary Electrophoretic Assays for Disaccharide Composition Analysis of Galactosaminoglycans in Biologic Samples and Drugs/Nutraceuticals. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_13

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

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