Abstract
Proteins that interact with the human kappa opioid receptor (hKOPR) may contribute to regulation and signaling of the receptor. In this paper, we focus on the protein 14-3-3zeta that regulates anterograde transport of the hKOPR from the endoplasmic reticulum (ER) to the Golgi apparatus. 14-3-3zeta interacts with the C-terminal domain of the receptor and promotes cell surface expression of the hKOPR by inhibiting coatomer protein I (COPI) and RVR motif-mediated ER retension of the hKOPR. Here we describe three experimental procedures we used to evaluate the interaction between hKOPR and 14-3-3zeta: co-immunoprecipitation, pull-down assay and immunofluorescence microscopy.
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References
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Acknowledgments
This work was supported by the National Institutes of Health (grant numbers R01 DA017302, R03 DA036802 and P30 DA013429).
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Chen, C., Huang, P., Liu-Chen, LY. (2015). Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR). In: Spampinato, S. (eds) Opioid Receptors. Methods in Molecular Biology, vol 1230. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1708-2_10
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DOI: https://doi.org/10.1007/978-1-4939-1708-2_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1707-5
Online ISBN: 978-1-4939-1708-2
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