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Agrobacterium Protocols pp 101–117Cite as

Maize (Zea mays L.)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.

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Acknowledgments

The authors thank Hanna Sundberg and Grace Beckett for their assistance in the greenhouse and lab and Aaron Brand for his help with greenhouse care and maintenance. This project was supported in part by the USDA National Institute of Food and Agriculture, Hatch project number # IOW05162, by the State of Iowa funds, and the National Science Foundation (DBI-0923827).

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Authors and Affiliations

  1. Center for Plant Transformation, Plant Sciences Institute, and Department of Agronomy, Iowa State University, 2104 Agronomy Hall, Ames, IA, 50011-1010, USA

    Bronwyn Frame, Katey Warnberg, Marcy Main & Kan Wang

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  1. Bronwyn Frame
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  2. Katey Warnberg
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  3. Marcy Main
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  4. Kan Wang
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Corresponding author

Correspondence to Bronwyn Frame .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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Frame, B., Warnberg, K., Main, M., Wang, K. (2015). Maize (Zea mays L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_8

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

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