Abstract
Medicago truncatula has been developed into a model species for legumes. The M. truncatula genotype Jemalong A17 has been used for EST and genome sequencing. However, this genotype is difficult to regenerate from callus cultures. By using cotyledons as explants for Agrobacterium infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from Jemalong A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3–4 months after Agrobacterium-mediated transformation. Transformation frequency was in the range of 5–12 %.
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References
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Wright, E., Wang, ZY. (2015). Medicago truncatula Transformation Using Cotyledonary Explants. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_3
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DOI: https://doi.org/10.1007/978-1-4939-1695-5_3
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Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-1694-8
Online ISBN: 978-1-4939-1695-5
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