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Sugar Beet (Beta vulgaris L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentiation, callus proliferation, and regeneration. Because of the infrequent occurrence of such a genotype in sugar beet populations, our protocol includes an in vitro propagation method for germplasm preservation. The starting materials for transgenic experiments are aseptic shoots grown from surface-sterilized seed balls. Callus is induced from leaf explants and subsequently infected with Agrobacterium. Plantlets are regenerated from transgenic callus and vernalized for flowering, if necessary. The efficiency of transformation was quite high; in our laboratory, the culture of only ten leaf explants, on average, generated one transgenic plant.

The original version of this chapter was revised: See the “Erratum” chapter near the end of the book for a complete listing of the revisions

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-1695-5_29

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Acknowledgments

The authors wish to thank Ms. Chisato Osawa for her technical assistance. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan; Grants-in-Aid for science and technology research program for agriculture, forestry, fisheries and food industry, Japan; and Grants-in-Aid for Scientific Research from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

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Authors and Affiliations

  1. Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

    Hiroyo Kagami, Masayuki Kurata, Tetsuo Mikami & Tomohiko Kubo

  2. Hokkaido Agricultural Research Center (HARC), National Agriculture and Food Research Organization (NARO), Sapporo, 062-8555, Japan

    Hiroaki Matsuhira

  3. Memuro Upland Farming Research Station, HARC/NARO, Memuro, 082-0071, Japan

    Kazunori Taguchi

  4. Central Agricultural Experiment Station, Agriculture Research Department, Hokkaido Research Organization, Takikawa, 073-0013, Japan

    Hideto Tamagake

Authors
  1. Hiroyo Kagami
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  2. Masayuki Kurata
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  3. Hiroaki Matsuhira
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  4. Kazunori Taguchi
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  5. Tetsuo Mikami
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  6. Hideto Tamagake
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  7. Tomohiko Kubo
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Corresponding author

Correspondence to Tomohiko Kubo .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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Kagami, H. et al. (2015). Sugar Beet (Beta vulgaris L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_27

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_27

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

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