Abstract
Pepper is a recalcitrant plant for Agrobacterium-mediated genetic transformation. Several obstacles to genetic transformation remain such as extremely low transformation rates; the choice of correct genotype is critical; and there is a high frequency of false positives due to direct shoot formation. Here, we report a useful protocol with a suitable selection method. The most important aspect of the pepper transformation protocol is selecting shoots growing from the callus, which is referred to as callus-mediated shoot formation. This protocol is a reproducible and reliable system for pepper transformation.
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Acknowledgment
This study was supported by a grant from the Next-Generation BioGreen21 research fund of the Rural Development of Administration from the Republic of Korea (PJ008178012014).
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Min, J., Shin, S.H., Jeon, E.M., Park, J.M., Hyun, J.Y., Harn, C.H. (2015). Pepper, Chili (Capsicum annuum). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_25
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DOI: https://doi.org/10.1007/978-1-4939-1695-5_25
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