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Clover, Red (Trifolium pratense)

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Book cover Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Genetic modification of plants by the insertion of transgenes can be a powerful experimental approach to answer basic questions about gene product function. This technology can also be used to make improved crop varieties for use in the field. To apply this powerful tool to red clover, an important forage legume, a population of red clover with high potential for regeneration in tissue culture has been developed. Here we provide a detailed procedure for Agrobacterium-mediated transformation of genotypes derived from this regenerable population. We have successfully used this methodology to express β-glucuronidase (GUS) reporter genes as well as for hairpin RNA-mediated silencing of endogenous genes for polyphenol oxidase and a transferase crucial in phaselic acid accumulation.

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Acknowledgements

We wish to thank Heather Green for helpful comments on the manuscript, Sara Zerbel and Lisa Koch for excellent technical assistance, and Dave Rancour for photographic assistance.

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Authors and Affiliations

  1. Agricultural Research Service, U.S. Department of Agriculture, U.S. Dairy Forage Research Center, 1925 Linden Drive West, Madison, WI, 53706, USA

    Michael L. Sullivan Ph.D.

  2. Department of Agronomy, University of Florida, Gainesville, FL, USA

    Kenneth H. Quesenberry Ph.D.

Authors
  1. Michael L. Sullivan Ph.D.
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  2. Kenneth H. Quesenberry Ph.D.
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Corresponding author

Correspondence to Michael L. Sullivan Ph.D. .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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Sullivan, M.L., Quesenberry, K.H. (2015). Clover, Red (Trifolium pratense). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_19

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

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