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Alfalfa (Medicago sativa L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Alfalfa (Medicago sativa L.) is a high-quality forage crop widely grown throughout the world. This chapter describes an efficient protocol that allows for the generation of large number of transgenic alfalfa plants by sonication-assisted Agrobacterium-mediated transformation. Binary vectors carrying different selectable marker genes that confer resistance to phosphinothricin (bar), kanamycin (npt II), or hygromycin (hph) were used to generate transgenic alfalfa plants. Intact trifoliates collected from clonally propagated plants in the greenhouse were sterilized with bleach and then inoculated with Agrobacterium strain EHA105. More than 80 % of infected leaf pieces could produce rooted transgenic plants in 4–5 months after Agrobacterium-mediated transformation.

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References

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Acknowledgments

We thank Yewei Wang and Steven Tudor for technical assistance in alfalfa transformation at the Samuel Roberts Noble Foundation.

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Authors and Affiliations

  1. Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA

    Chunxiang Fu, Timothy Hernandez, Chuanen Zhou & Zeng-Yu Wang

Authors
  1. Chunxiang Fu
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  2. Timothy Hernandez
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  3. Chuanen Zhou
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  4. Zeng-Yu Wang
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Corresponding author

Correspondence to Zeng-Yu Wang .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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© 2015 Springer Science+Business Media New York

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Fu, C., Hernandez, T., Zhou, C., Wang, ZY. (2015). Alfalfa (Medicago sativa L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_17

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

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