Skip to main content
SpringerLink
Log in
Book cover

Agrobacterium Protocols pp 181–188Cite as

Sorghum (Sorghum bicolor)

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Agrobacterium-mediated transformation of sorghum (Sorghum bicolor L. Moench) targeting immature embryo explants is a route to introduce transgenic alleles into the crop. The protocol requires maintenance of quality stock plants under greenhouse conditions for a constant supply of immature embryo explants. This is typically carried out by a regular sowing of seeds, minimal use of pesticides, and monitoring of plants to document pollen dispersal and bagging of heads. The time frame from explant inoculation to establishment of a primary transgenic event in the greenhouse typically ranges from 4 to 6 months. Seed set in the primary transformants is comparable to greenhouse-grown stock plants, with the majority of the transgenic alleles being inherited as a single functional locus.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Paterson AH (2008) Genomics of sorghum. Int J Plant Genomics 2008:6

    Article  Google Scholar 

  2. Paterson AH et al (2009) The Sorghum bicolor genome and the diversification of grasses. Nature 457:551

    Article  CAS  PubMed  Google Scholar 

  3. Cai T, Pierce DA, Tagliani LA, Zhao Z-Y (2002) Sorghum transformation, Pioneer Hi-Bred International, Inc, United States Patent 6,396,298

    Google Scholar 

  4. Battraw M, Hall TC (1991) Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase-II and ß-glucuronidase genes. Theor Appl Genet 82:161

    Article  CAS  PubMed  Google Scholar 

  5. Gao Z, Xie X, Ling Y, Muthukrishnan S, Liang GH (2005) Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system. Plant Biotechnol J 3:591

    Article  CAS  PubMed  Google Scholar 

  6. Gurel S et al (2009) Efficient, reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos. Plant Cell Rep 28:429

    Article  CAS  PubMed  Google Scholar 

  7. Zhao ZY et al (2000) Agrobacterium-mediated sorghum transformation. Plant Mol Biol 44:789

    Article  CAS  PubMed  Google Scholar 

  8. Nguyen T-V, Thu TT, Claeys M, Angenon G (2007) Agrobacterium-mediated transformation of sorghum (Sorghum bicolor (L.) Moench) using an improved in vitro regeneration system. Plant Cell Tiss Org Cult 91:155

    Article  CAS  Google Scholar 

  9. Howe A, Sato S, Dweikat I, Fromm M, Clemente T (2006) Rapid and reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell Rep 25:784

    Article  CAS  PubMed  Google Scholar 

  10. Kumar T, Howe A, Sato S, Dweikat I, Clemente T (2012) In: Paterson AH (ed) Genetics and genomics of the saccharinae, vol 11, Springer, New York, NY, pp 205–221

    Google Scholar 

  11. Fraley RT et al (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci U S A 80:4803

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Benfey PN, Chua NH (1990) The cauliflower mosaic virus-35s promoter – combinatorial regulation of transcription in plants. Science 250:959

    Article  CAS  PubMed  Google Scholar 

  13. Hajdukiewicz P, Svab Z, Maliga P (1994) The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol 25:989

    Article  CAS  PubMed  Google Scholar 

  14. Miller FR (1984) Registration of RTx430 sorghum parental line. Crop Sci 24:1224

    Article  Google Scholar 

  15. Palanichelvam K et al (2000) A second T-region of the soybean-supervirulent chrysopine-type Ti plasmid pTiChry5, and construction of a fully disarmed vir helper plasmid. Mol Plant Microbe Interact 13:1081

    Article  CAS  PubMed  Google Scholar 

  16. Hood EE, Helmer GL, Fraley RT, Chilton M-D (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol 168:1291

    PubMed Central  CAS  PubMed  Google Scholar 

  17. Chilton M-D et al (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci U S A 71:3672

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Elkonin LA, Pakhomova NV (2000) Influence of nitrogen and phosphorus on induction embryogenic callus of sorghum. Plant Cell Tiss Org Cult 61:115

    Article  Google Scholar 

  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agronomy and Horticulture, Center for Plant Science Innovation, George W. Beadle Center, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE, 68588-0665, USA

    Xiaomei Guo, Zhengxiang Ge & Tom E. Clemente

  2. Department of Agronomy and Horticulture, Center for Plant Science Innovation, George W. Beadle Center, Center for Biotechnology, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE, 68588-0665, USA

    Shirley J. Sato

Authors
  1. Xiaomei Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhengxiang Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shirley J. Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tom E. Clemente
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tom E. Clemente .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Guo, X., Ge, Z., Sato, S.J., Clemente, T.E. (2015). Sorghum (Sorghum bicolor). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_14

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation