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Rice, Indica (Oryza sativa L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Indica varieties, which are generally recalcitrant to tissue culture and transformation, occupy 80 % of rice cultivation area in the world. Therefore, transformation method for indica rice must be improved greatly so that global rice production would take full advantage of cutting-edge biotechnology. An efficient protocol for indica transformation mediated by Agrobacterium tumefaciens is hereby described. Immature embryos collected from plants in a greenhouse are cocultivated with A. tumefaciens after pretreatment with heat and centrifuging. The protocol was successfully tested in many elite indica cultivars such as IR8, IR24, IR58025B, IR64, IR72, Suweon 258, and Nanjing 11, yielding between 5 and 15 of independent transgenic plants per immature embryo. The use of immature embryos is recommended because gene transfer to them could be much more efficient and much less genotype dependent than gene transfer to callus.

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Acknowledgment

We thank Ms. Chiaki Sakuma and Ms. Tomoko Sakai for technical assistance.

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Authors and Affiliations

  1. Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka, 438-0802, Japan

    Yukoh Hiei, Yuji Ishida & Toshihiko Komari

Authors
  1. Yukoh Hiei
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  2. Yuji Ishida
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  3. Toshihiko Komari
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Corresponding author

Correspondence to Yukoh Hiei .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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Hiei, Y., Ishida, Y., Komari, T. (2015). Rice, Indica (Oryza sativa L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_12

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

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