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PET Imaging for Tyrosine Kinase Inhibitor (TKI) Biodistribution in Mice

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Book cover Apoptosis and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1219))

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Abstract

Receptor tyrosine kinases play a critical role in cell growth, survival, and proliferation, and are considered potential molecular targets for the treatment of cancer. Although several tyrosine kinase inhibitors (TKIs), such as erlotinib and gefitinib, have demonstrated clinical efficacy via the inhibition of the epidermal growth factor receptor (EGFR), most TKIs are only effective in a small proportion of patients. Positron emission tomography (PET) imaging is a methodology of molecular imaging based on nuclear imaging. PET imaging in combination with radiolabeled TKIs improves accuracy of quantitative imaging strategies and the probability of successful drug development, and may facilitate the stratification of patients. Here, we describe a protocol for PET imaging using radiolabeled TKI in preclinical trials.

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Author information

Authors and Affiliations

  1. Bioimaging Research Laboratories, Astellas Pharma Inc., 21 Miyukigaoka, Tsukuba, Ibaraki, 305-8585, Japan

    Hiroshi Fushiki Ph.D., Yoshihiro Murakami, Sosuke Miyoshi & Shintaro Nishimura

Authors
  1. Hiroshi Fushiki Ph.D.
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  2. Yoshihiro Murakami
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  3. Sosuke Miyoshi
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  4. Shintaro Nishimura
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Corresponding author

Correspondence to Hiroshi Fushiki Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, Connecticut, USA

    Gil Mor

  2. Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, Connecticut, USA

    Ayesha B. Alvero

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Fushiki, H., Murakami, Y., Miyoshi, S., Nishimura, S. (2015). PET Imaging for Tyrosine Kinase Inhibitor (TKI) Biodistribution in Mice. In: Mor, G., Alvero, A. (eds) Apoptosis and Cancer. Methods in Molecular Biology, vol 1219. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1661-0_15

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  • DOI: https://doi.org/10.1007/978-1-4939-1661-0_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1660-3

  • Online ISBN: 978-1-4939-1661-0

  • eBook Packages: Springer Protocols

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