Abstract
Apoptosis can be measured by number of methods by taking advantage of the morphological, biochemical, and molecular changes undergoing in a cell during this process. The best recognized biochemical hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fluorochrome substrates, as well as employing the fluorescein-labeled CaspaTag pan-caspase in situ detection kit.
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Acknowledgments
This work was supported by a research grant from the National Institutes of Health to A.A. (CA127264).
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Choudhary, G.S., Al-harbi, S., Almasan, A. (2015). Caspase-3 Activation Is a Critical Determinant of Genotoxic Stress-Induced Apoptosis. In: Mor, G., Alvero, A. (eds) Apoptosis and Cancer. Methods in Molecular Biology, vol 1219. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1661-0_1
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DOI: https://doi.org/10.1007/978-1-4939-1661-0_1
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