Abstract
The efficacy of Agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6 %) using a novel encapsulation-based, antibiotic selection procedure. The detailed protocol using a standard plant transformation vector (pCAMBIA1304) as reported in an ‘elite’ Indian variety (Queen) of pineapple [Ananas comosus (L.) Merr] can be applied to other varieties of pineapple for introgression of target genes.
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Acknowledgment
A critical reading of the manuscript by Ms. Ranjana Prasad is duly acknowledged. Authors are thankful to the director of Bose Institute.
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Gangopadhyay, G., Mukherjee, K.K. (2015). Pineapple [Ananas comosus (L.) Merr.]. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_23
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DOI: https://doi.org/10.1007/978-1-4939-1658-0_23
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