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Agrobacterium Protocols pp 111–119Cite as

Apricot (Prunus armeniaca L.)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

Abstract

A protocol for Agrobacterium-mediated stable transformation of whole leaf explants of the apricot (Prunus armeniaca) cultivars ‘Helena’ and ‘Canino’ is described. Regenerated buds were selected using a two-step selection strategy with paromomycin sulfate and transferred to bud multiplication medium 1 week after they were detected for optimal survival. After buds were transferred to bud multiplication medium, antibiotic was changed to kanamycin and concentration increased gradually at each transfer to fresh medium in order to eliminate possible escapes and chimeras. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines, was 5.6 %. Green and healthy buds, surviving high kanamycin concentration, were transferred to shoot multiplication medium where they elongated in shoots and proliferated. Elongated transgenic shoots were rooted in a medium containing 70 μM kanamycin. Rooted plants were acclimatized following standard procedures. This constitutes the only transformation protocol described for apricot clonal tissues and one of the few of Prunus.

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Authors and Affiliations

  1. Grupo de Biotecnología de Frutales, Departamento de Mejora, CEBAS-CSIC, Aptd. de Correos 164, 30.100, Murcia, Spain

    César Petri, Nuria Alburquerque & Lorenzo Burgos

Authors
  1. César Petri
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  2. Nuria Alburquerque
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  3. Lorenzo Burgos
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Corresponding author

Correspondence to Lorenzo Burgos .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Petri, C., Alburquerque, N., Burgos, L. (2015). Apricot (Prunus armeniaca L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_10

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

  • eBook Packages: Springer Protocols

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